Embryonic stem cells derived from C57BL/6J and C57BL/6N mice

Area of Science:

• Developmental Biology
• Stem Cell Biology
• Genetics

Background:

• C57BL/6 embryonic stem (ES) cells are crucial for generating knockout mice without extensive backcrossing.
• Publicly available C57BL/6 ES cell lines with confirmed pluripotency post-homologous recombination are scarce.
• Facilitating research requires accessible C57BL/6 ES cell lines for biological and medical applications.

Purpose of the Study:

• To establish efficient methods for deriving C57BL/6 ES cell lines.
• To produce germline chimeric mice from these C57BL/6 ES cells.
• To make C57BL/6 ES cell lines available for research.

Main Methods:

• Utilized knockout serum replacement as a medium supplement for ES cell culture.
• Employed 8-cell blastomeres as recipient embryos for chimera production.
• Established numerous ES cell lines from C57BL/6J and C57BL/6N blastocysts.

Main Results:

• Successfully established multiple ES cell lines from both C57BL/6J and C57BL/6N strains.
• The majority of derived ES cell lines exhibited normal chromosome numbers.
• Germline chimeric mice were generated from both C57BL/6J and C57BL/6N ES cells.
• Gene knockout mice were successfully produced using the B6J-S1UTR ES cell line.

Conclusions:

• Developed effective protocols for establishing C57BL/6 ES cell lines and generating chimeric mice.
• Provided valuable C57BL/6 ES cell lines (B6J-S1UTR, B6J-23UTR, B6N-22UTR) for the research community.
• These resources significantly aid in the generation of knockout mouse models for biological and medical research.