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Related Concept Videos

Fixation and Sectioning01:03

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...

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Related Experiment Video

Updated: Jul 2, 2026

Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue
09:20

Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue

Published on: September 2, 2013

[A novel fixed paraffin-embedded tissue processing for proteomic analysis].

V Bellet1, P Rochaix, A Mange

  • 1Laboratoire de biologie cellulaire et hormonale, Hôpital Arnaud de Villeneuve, CHU de Montpellier.

Annales De Biologie Clinique
|August 30, 2008
PubMed
Summary
This summary is machine-generated.

A new non-toxic tissue fixative preserves protein integrity, offering an alternative to formalin-fixed, paraffin-embedded (FFPE) tissues for proteomic studies and diagnostics.

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A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions
09:00

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions

Published on: April 18, 2025

Related Experiment Videos

Last Updated: Jul 2, 2026

Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue
09:20

Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue

Published on: September 2, 2013

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions
09:00

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions

Published on: April 18, 2025

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Pathology

Context:

  • Human tissues are crucial for biomarker discovery and therapeutic target identification.
  • Formalin-fixed, paraffin-embedded (FFPE) tissues are abundant archival resources but present challenges for proteomic analysis due to protein crosslinking.

Purpose:

  • To evaluate a novel, non-toxic tissue fixative for proteomic investigations.
  • To compare protein preservation and analysis feasibility with frozen and FFPE tissues.

Summary:

  • The new fixative demonstrated comparable protein patterns to frozen tissues using electrophoresis and mass spectrometry.
  • Western blot and immunohistochemistry analyses showed successful protein detection and comparable results between the new fixative and frozen tissues.

Impact:

  • This new protocol provides an accessible alternative to FFPE, supporting both diagnostic pathology and advanced tissue proteomic research.
  • Facilitates deeper molecular insights from archival and fresh tissue samples.