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Related Experiment Videos

Immunoreactive endothelin in human kidney.

S Morita1, K Kitamura, Y Yamamoto

  • 1First Department of Internal Medicine, Miyazaki Medical College, Japan.

Annals of Clinical Biochemistry
|May 1, 1991
PubMed
Summary
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This study found high concentrations of endothelin-1 in human kidney inner medulla. This differs from rat and pig kidneys, suggesting species-specific endothelin gene expression.

Area of Science:

  • Nephrology
  • Endocrinology
  • Molecular Biology

Background:

  • Endothelin (ET) is a potent vasoconstrictor peptide.
  • The presence and specific forms of endothelin in human kidney tissue are not fully elucidated.

Purpose of the Study:

  • To quantify and characterize immunoreactive endothelin in human kidney inner medulla.
  • To compare endothelin expression in human kidney with that of rat and pig.

Main Methods:

  • Radioimmunoassay (RIA) was used to measure immunoreactive endothelin.
  • Reverse-phase high-performance liquid chromatography (RP-HPLC) was employed for characterization.

Main Results:

  • High concentrations of immunoreactive endothelin were detected in human kidney inner medulla from both necropsy and nephrectomy samples.

Related Experiment Videos

  • RP-HPLC analysis confirmed that the predominant, if not sole, endothelin isopeptide in human kidney inner medulla is endothelin-1.
  • In contrast, rat and pig kidney inner medulla contain both endothelin-1 and endothelin-3.

    • Conclusions:

    • Human kidney inner medulla expresses high levels of endothelin-1.
    • Species-specific differences exist in endothelin isopeptide expression within the kidney medulla.