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Cloning of alginate lyase gene (alxM) and expression in Escherichia coli
B J Brown1, J F Preston, L O Ingram
1Department of Chemistry, Michigan Technological University, Houghton 49931.
Applied and Environmental Microbiology
|June 1, 1991
Abstract:
The alxM gene encoding a D-mannuronan-specific alginate lyase has been cloned from a marine bacterium isolated as an epiphyte on the brown alga, Sargassum fluitans. Expression of this gene in Escherichia coli provides a source of this enzyme for probing alginate structure and modifying the mannuronan-rich alginate polymers produced by bacterial pathogens.