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Allosteric Proteins-ATCase01:19

Allosteric Proteins-ATCase

Binding sites linkages can regulate a protein's function.  For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
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Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
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An Assay for Quantifying Protein-RNA Binding in Bacteria
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Published on: June 12, 2019

An ABA-binding protein with nucleic acid-binding property.

Z Zheng1, Z Jin, X Zhou

  • 1Agronomy Department, Nanjing Agricultural University, 210095, Nanjing, China.

Science in China. Series C, Life Sciences
|August 30, 2008
PubMed
Summary
This summary is machine-generated.

Researchers screened a maize cDNA library for ABA-binding proteins (ABBP). Unexpectedly, they found the purified ABBP and its antibodies recognized RNA, suggesting ABBP binds nucleic acids and forms complexes with rRNA.

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Area of Science:

  • Molecular Biology
  • Plant Biochemistry

Background:

  • ABA-binding proteins (ABBP) are crucial in plant hormone signaling.
  • Screening maize cDNA libraries can identify novel protein interactions.

Purpose of the Study:

  • To identify novel ABA-binding proteins (ABBP) in maize using antibody screening.
  • To investigate the biochemical properties of the identified ABBP and its interactions.

Main Methods:

  • Maize cDNA expression library screening with antibodies against ABBP.
  • Antibody characterization and protein purification via ABA affinity chromatography.
  • Analysis of purified ABBP and derived antibodies for nucleic acid binding.

Main Results:

  • One cDNA clone coding for a polypeptide with homology to nucleic acid-binding proteins was identified.
  • Unexpectedly, approximately 120 cDNA clones coding for maize 17s rRNA were obtained.
  • Purified ABBP and its antibodies demonstrated the presence of rRNA and recognized both RNA and ABBP, indicating a complex formation.

Conclusions:

  • The identified ABBP possesses nucleic acid-binding properties.
  • ABBP can form complexes with ribosomal RNA (rRNA).
  • This finding suggests a potential novel role for ABBP beyond ABA signaling, involving RNA interactions.