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Updated: Jul 2, 2026

Identification of Functional Protein Regions Through Chimeric Protein Construction
Published on: January 8, 2019
Identification of a novel DNA-binding protein to osmotin promoter
Abstract:
One novel osmotin promoter, binding-protein (OPBP1) gene, was isolated from salt-adapted tobacco suspension cells using yeast one-hybrid system. The OPBP1 interacted specifically in vivo with FA, a DNA sequence from the 5 upstream region of osmotin gene, which was essential for osmotin responsiveness. The deduced amino acid sequence of OPBP1 contained a conserved motif of a new gene family, AP2 family. This protein did not contain the typical motif found in the most known DNA-binding proteins and transcription factors.
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