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Updated: Jul 2, 2026

Glucose Uptake Measurement and Response to Insulin Stimulation in In Vitro Cultured Human Primary Myotubes
Published on: June 25, 2017
Insulin and insulin mutants stimulate glucose uptake in rat adipocytes
Abstract:
A simple method to determine the in vitro biological activity of insulin by measuring glucose uptake in the rat adipocytes is presented here. In the presence of insulin, the glucose uptake is 5-6 times more than the basal control. And the uptake of D-[3-(3)H]-glucose is linear as the logarithm of insulin concentration from 0.2 microg/L to 1.0 microg/L. Glucose and 3-O-methyl-glucose inhibit D-[3-(3)H]-glucose uptake into adipocytes. By this method, the in vitro biological activity of [B(2)-Lys]-insulin and [B(3)-Lys]-insulin was measured to be 61.6% and 154% respectively, relative to that of insulin.
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