Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jul 2, 2026

Isolation and Activation of Murine Lymphocytes

Isolation and Activation of Murine Lymphocytes

Published on: October 30, 2016

Surface markers for the murine oval cell response.

Craig Dorrell¹, Laura Erker, Kelsea M Lanxon-Cookson

¹Oregon Health & Science University and the Oregon Stem Cell Center, Portland, OR 97239, USA. dorrellc@ohsu.edu

Hepatology (Baltimore, Md.)

|August 30, 2008

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

RAAVioli: A comprehensive approach to characterizing AAV vector integrations and rearrangements.

Molecular therapy. Advances·2026

Same author

A clinically relevant retrograde intraductal injection (RIDI) for lipid nanoparticles-mediated base editing in the pancreas.

Nanomedicine : nanotechnology, biology, and medicine·2026

Same author

A porcine model of Fanconi anemia.

PloS one·2025

Same author

Cell networks in the mouse liver during partial hepatectomy.

Stem cell reports·2025

Same author

One Shock, Not One Cure: Electroporation Reveals Disease-Specific Constraints in Hepatocyte Gene Editing Therapy.

Biology·2025

Same author

Glucagon and GLP-1 Accelerate Pseudo-Islet Assembly and Unmask Sex-Specific Islet Fragmentation Dynamics.

bioRxiv : the preprint server for biology·2025

Researchers developed new monoclonal antibodies to identify and isolate liver progenitor cells in mice. These tools aid in studying liver cell biology and progenitor activation, advancing regenerative medicine research.

Area of Science:

Hepatology and stem cell biology
Immunology and reagent development

Background:

Liver progenitor cell activation is crucial for liver regeneration and disease.
Studying hepatic subpopulations is challenging due to limited specific reagents.
Mouse models offer powerful genetic tools but lack specific cell isolation methods.

Purpose of the Study:

To generate novel monoclonal antibodies for identifying and isolating mouse liver progenitor cells.
To develop reagents for studying the oval cell response and hepatic cell subpopulations.
To facilitate research into ductal and periductal hepatic cell biology.

Main Methods:

Immunization of Fischer rats with nonparenchymal liver cells from mice treated with 3,5-diethoxycarbonyl-1,4-dihydrocollidine.
Generation of monoclonal antibodies targeting surface antigens on liver cell subsets.

More Related Videos

Rapid and Specific Immunomagnetic Isolation of Mouse Primary Oligodendrocytes

Rapid and Specific Immunomagnetic Isolation of Mouse Primary Oligodendrocytes

Published on: May 21, 2018

Murine Lymphocyte Labeling by 64Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT

Murine Lymphocyte Labeling by ⁶⁴Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT

Published on: April 29, 2017

Related Experiment Videos

Last Updated: Jul 2, 2026

Isolation and Activation of Murine Lymphocytes

Isolation and Activation of Murine Lymphocytes

Published on: October 30, 2016

Rapid and Specific Immunomagnetic Isolation of Mouse Primary Oligodendrocytes

Rapid and Specific Immunomagnetic Isolation of Mouse Primary Oligodendrocytes

Published on: May 21, 2018

Murine Lymphocyte Labeling by 64Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT

Murine Lymphocyte Labeling by ⁶⁴Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT

Published on: April 29, 2017

Utilizing fluorescence-activated cell sorting (FACS) for viable cell subpopulation isolation.

Main Results:

A new panel of surface-reactive monoclonal antibodies was successfully generated.
These antibodies recognize distinct liver cell subsets, including those involved in oval cell response.
Antibodies enabled viable isolation of specific hepatic subpopulations via FACS.
Differential antibody activity was observed on normal and activated oval cells.

Conclusions:

A novel panel of monoclonal antibodies for investigating the murine oval cell response has been developed.
These reagents provide new tools for the identification and isolation of liver progenitor cells.
The developed antibodies will advance the study of hepatic cell biology and progenitor activation.