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Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling
Published on: March 20, 2018
Carcinogen adsorbent prepared from DNA complex by gamma-ray irradiation
Kazuya Furusawa1, Erika Kita, Toshihiko Saheki
1Department of Chemistry and Chemical Biology, Faculty of Engineering, Gunma University, Kiryu, Gunma, Japan.
Journal of Biomaterials Science. Polymer Edition
|August 30, 2008
Summary
Gamma-ray irradiation effectively cross-links DNA with various agents, forming gels for carcinogen adsorption. Optimal conditions were determined for creating insolubilized DNA gels, showing potential for novel adsorbent materials.
Area of Science:
- Biomaterials Science
- Radiation Chemistry
- Biopolymer Engineering
Background:
- DNA's potential for novel applications is limited by its solubility.
- Developing methods to insolubilize DNA is crucial for creating advanced materials.
- Carcinogen adsorption presents a significant environmental and health challenge.
Purpose of the Study:
- To investigate gamma-ray irradiation effects on DNA for insolubilization.
- To prepare novel DNA-based gels for carcinogen adsorption.
- To determine optimal conditions for homogeneous DNA gel formation.
Main Methods:
- Irradiation of aqueous DNA solutions and mixtures with additives (CoCl2, gelatin, BSA, CM-chitosan, CMC, Catinal).
- Preparation of cross-linked DNA precipitates and gels using gamma radiation.
- Characterization of gel properties: gel fraction and swelling ratio.
- Adsorption studies using acridine orange as a model carcinogen.
Main Results:
- Cross-linked DNA precipitates formed with CoCl2 at low doses (~10 Gy).
- Homogeneous DNA gels prepared from mixtures with gelatin, BSA, CMC, and Catinal within specific irradiation dose ranges.
- Gel properties (fraction, swelling) were quantified.
- Effective adsorption of acridine orange by the prepared DNA gels was observed.
Conclusions:
- Gamma-ray irradiation is a viable method for insolubilizing DNA.
- DNA-based gels can be tailored for carcinogen adsorption applications.
- Optimal conditions for producing homogeneous DNA gels were established, paving the way for novel adsorbent development.
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