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Genome-wide Profiling of Transcription Factor-DNA Binding Interactions in Candida albicans: A Comprehensive CUT&RUN Method and Data Analysis Workflow

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An alternative Candida spp. cell wall disruption method using a basic sorbitol lysis buffer and glass beads.

Crystale Siew Ying Lim¹, Chee Hong Tung, Rozita Rosli

¹Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, University Putra Malaysia, Serdang, Selangor, Malaysia.

Journal of Microbiological Methods

|August 30, 2008

Summary

A new, affordable method efficiently disrupts yeast cell walls using glass beads and sorbitol lysis buffer. This technique yields high-quality RNA and DNA for molecular biology applications.

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Published on: October 21, 2010

Area of Science:

Microbiology
Molecular Biology
Biotechnology

Background:

Effective cell wall disruption is crucial for isolating nucleic acids from yeast.
Existing methods can be expensive or complex.
Candida species present a challenge due to their robust cell walls.

Purpose of the Study:

To develop a cost-effective and simple method for yeast cell wall disruption.
To optimize cell lysis for subsequent nucleic acid isolation.
To provide a reliable technique for Candida spp. research.

Main Methods:

Utilized a modified cell wall disruption technique.
Employed glass beads in a sorbitol lysis buffer.
Integrated the method with commercial RNA and genomic DNA isolation kits.

Main Results:

Achieved efficient cell wall disruption in Candida spp.
Obtained high-quality RNA and genomic DNA.
Demonstrated the cost-effectiveness and simplicity of the protocol.

Conclusions:

The described method offers a practical solution for yeast cell lysis.
This technique facilitates high-yield nucleic acid extraction from Candida.
The protocol is suitable for various molecular applications requiring pure RNA or DNA.