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Experimental study on cell self-sealing during sonoporation.

Fang Yang1, Ning Gu, Di Chen

  • 1Jiangsu Laboratory for Biomaterials and Devices, State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China.

Journal of Controlled Release : Official Journal of the Controlled Release Society
|August 30, 2008
PubMed
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Ultrasound and microbubbles create temporary pores in breast cancer cells, allowing fluid uptake and triggering self-repair. This reparable sonoporation process involves cell membrane fusion with LAMP-1.

Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Oncology

Background:

  • Sonoporation utilizes ultrasound to temporarily increase cell membrane permeability.
  • Encapsulated microbubbles (EMBs) enhance ultrasound-induced effects on cells.
  • Understanding cellular repair mechanisms post-sonoporation is crucial for therapeutic applications.

Purpose of the Study:

  • To investigate reparable sonoporation in human breast cancer cells using moderate ultrasound and EMBs.
  • To elucidate the mechanism of cell membrane self-sealing following sonoporation.
  • To identify the molecular players involved in the cellular repair process.

Main Methods:

  • Exposure of human breast cancer cells to moderate ultrasound (0.25 MPa, 1 MHz) with EMBs for up to 40 seconds.

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  • Analysis of cell membrane integrity and pore formation using fluorescent microscopy and scanning electron microscopy.
  • Quantification of protein content in cell supernatant via Bradford assay to assess cellular response.

    • Main Results:

    • Reparable sonoporation was successfully achieved, enabling extracellular fluid uptake.
    • Oscillating EMBs generated shear stress, inducing transient pores in cell membranes.
    • Cellular self-sealing of pores was observed during the sonoporation process.
    • Lysosomal-associated membrane protein 1 (LAMP-1) was identified as potentially involved in membrane repair through fusion with the plasma membrane.

    Conclusions:

    • Moderate ultrasound combined with EMBs offers a method for reparable sonoporation in breast cancer cells.
    • The cellular self-sealing mechanism appears to involve LAMP-1, facilitating membrane repair under sonoporation stress.
    • This study provides insights into the cellular response to sonoporation, paving the way for potential therapeutic advancements.