Related Experiment Video
Updated: Jul 2, 2026

Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering (SEC-MALS)
Published on: June 20, 2019
Determination of membrane protein molecular weight using sedimentation equilibrium analytical ultracentrifugation
1Thomas C. Jenkins Department of Biophysics, Johns Hopkins University, Baltimore, Maryland.
Abstract:
Both the stoichiometry and stability of native membrane protein complexes pose challenges to understanding the biology of these proteins. Sedimentation equilibrium analytical ultracentrifugation is well recognized as a thermodynamically rigorous technique for determining these quantities for macromolecules. This unit describes the experimental strategies that can be used to extract this information for membrane proteins reconstituted in vitro in detergent micelle or detergent/lipid mixed micelle solutions.
Related Concept Videos
Two-dimensional Gel Electrophoresis
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as cells...
Detergent Purification of Membrane Proteins
Determination of Molar Masses of Polymers II
SDS-PAGE
A variation of gel electrophoresis, termed polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...
Centrifugation

