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Related Experiment Video

Updated: Jul 2, 2026

Characterization of Cell Membrane Extensions and Studying Their Roles in Cancer Cell Adhesion Dynamics

Characterization of Cell Membrane Extensions and Studying Their Roles in Cancer Cell Adhesion Dynamics

Published on: March 26, 2018

Plakophilin3 downregulation leads to a decrease in cell adhesion and promotes metastasis.

Samrat T Kundu¹, Prajakta Gosavi, Nileema Khapare

¹ACTREC, Tata Memorial Centre, Kharghar Node, Navi Mumbai, India.

International Journal of Cancer

|August 30, 2008

Summary

Loss of plakophilin3 (a desmosomal protein) reduces cell adhesion, promoting cancer progression and metastasis. This study demonstrates plakophilin3

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Characterization of Cell Membrane Extensions and Studying Their Roles in Cancer Cell Adhesion Dynamics

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Conditional Knockdown of Gene Expression in Cancer Cell Lines to Study the Recruitment of Monocytes/Macrophages to the Tumor Microenvironment

Conditional Knockdown of Gene Expression in Cancer Cell Lines to Study the Recruitment of Monocytes/Macrophages to the Tumor Microenvironment

Published on: November 23, 2017

Area of Science:

Cell Biology
Oncology
Molecular Biology

Background:

Plakophilin3 (a desmosomal plaque protein) levels are decreased in aggressive oropharyngeal and colon tumors.
Reduced plakophilin3 expression correlates with invasive and metastatic cancers.

Purpose of the Study:

To investigate the role of plakophilin3 in neoplastic progression.
To determine if plakophilin3 loss stimulates cancer cell migration and metastasis.

Main Methods:

Plakophilin3 expression was inhibited using RNA interference in HCT116, HaCaT, and fetal buccal mucosa cell lines.
Cell-cell adhesion, migration, desmosome structure, and tumor formation in nude mice were assessed.

Main Results:

Plakophilin3 knockdown decreased cell-cell adhesion and increased cell migration.
Desmosome size was reduced, leading to increased colony formation and tumor growth.
HCT116 plakophilin3-knockdown cells showed accelerated tumor formation and lung metastasis in vivo.

Conclusions:

Plakophilin3 loss impairs desmosome function, reduces cell adhesion, and promotes neoplastic progression.
The findings support plakophilin3's role as a tumor suppressor and highlight its significance in metastasis.