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Related Concept Videos

Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

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The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach
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G2R Cre reporter transgenic zebrafish.

Shunichi Yoshikawa1, Koichi Kawakami, Xinping C Zhao

  • 1Department of Ophthalmology and Visual Science, University of Texas Health Science Center at Houston, Houston, Texas 77030, USA.

Developmental Dynamics : an Official Publication of the American Association of Anatomists
|August 30, 2008
PubMed
Summary

Researchers developed new zebrafish reporter lines to track Cre recombinase activity. These G2R fish change from green to red fluorescent protein, enabling precise gene manipulation and lineage tracing in zebrafish.

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Area of Science:

  • Genetics
  • Molecular Biology
  • Zebrafish Model Systems

Background:

  • The Cre/loxP system is crucial for in vivo DNA manipulation and gene function studies, primarily in mice.
  • Adapting powerful genetic tools like Cre/loxP for zebrafish research is essential for expanding model system utility.

Purpose of the Study:

  • To generate novel reporter transgenic zebrafish lines for visualizing Cre recombinase activity.
  • To facilitate the development of cell type-specific Cre transgenics and lineage tracing in zebrafish.

Main Methods:

  • Generation of G2R transgenic zebrafish expressing GFP and RFP fluorescent proteins.
  • Utilizing the ubiquitous Xenopus EF1 alpha promoter for gene expression.
  • Observing Cre-mediated recombination-induced color change from green to red.

Main Results:

  • Successful creation of G2R zebrafish lines that exhibit a distinct color switch upon Cre activity.
  • Demonstrated utility of G2R lines for detecting Cre recombination events in vivo.
  • Established a valuable tool for future genetic studies in zebrafish.

Conclusions:

  • The G2R zebrafish reporter lines provide an effective method for monitoring Cre recombinase activity.
  • These lines are instrumental for advancing cell type-specific gene targeting and lineage mapping in zebrafish research.