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Updated: Jul 2, 2026

Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues

Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues

Published on: June 21, 2018

A direct and rapid leaf water extraction method for isotopic analysis.

L I Peters¹, D Yakir

¹Department of Environmental Sciences and Energy Research, Weizmann Institute of Science, Rehovot, Israel Leon.Peters@weizmann.ac.il

Rapid Communications in Mass Spectrometry : RCM

|August 30, 2008

Summary

A new, rapid centrifugation/filtration method simplifies leaf water extraction for isotopic analysis. This technique significantly reduces processing time and costs, enabling large-scale ecophysiological and biogeochemical studies.

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Last Updated: Jul 2, 2026

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Area of Science:

Ecology
Biogeochemistry
Analytical Chemistry

Background:

Isotopic analysis of leaf water is crucial for understanding ecophysiological and biogeochemical processes.
Conventional methods (cryogenic vacuum or azeotrophic distillation) are time-consuming, labor-intensive, and require specialized equipment and toxic materials, limiting large-scale studies.

Purpose of the Study:

To develop and validate a rapid, cost-effective, and reliable method for extracting leaf water for isotopic analysis.
To overcome the analytical bottleneck associated with conventional leaf water extraction techniques.

Main Methods:

A novel technique involving centrifugation/filtration of pulverized leaf samples in their original tubes using a simple apparatus.
Leaf water extracts were analyzed using pyrolysis gas chromatography isotope ratio mass spectrometry (PYR/GC/IRMS).
Validation against cryogenic vacuum distillation was performed.

Main Results:

The new method significantly reduced extraction time from 4 hours to 45 minutes for an eight-sample subset.
Overall accuracy was +/-0.5 per thousand compared to cryogenic vacuum distillation over a 21 per thousand range.
Effects of soluble carbohydrates were minimal and correctable.

Conclusions:

This centrifugation/filtration method offers a rapid, low-cost, and reliable alternative to traditional distillation techniques.
The method facilitates high-throughput leaf water isotopic analysis, supporting broader ecosystem and global-scale research.