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Related Experiment Videos

In-vivo epidermal nuclear reactions: a selective process.

M S Koelle1, V Kumar, E H Beutner

  • 1Ernest Witebsky Center for Immunology, Department of Microbiology, University, Buffalo, SUNY 14214.

The British Journal of Dermatology
|July 1, 1991
PubMed
Summary

Epidermal nuclear reactions in skin biopsies are a genuine finding, not an artifact. This study shows IgG penetration into epidermal nuclei is an active, selective process.

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Area of Science:

  • Immunodermatology
  • Autoimmunity Research

Background:

  • Epidermal in-vivo nuclear reactions of Immunoglobulin G (IgG) are observed in patients with connective tissue diseases.
  • These reactions are linked to high titers of antibodies to ribonucleoprotein (RNP).

Purpose of the Study:

  • To determine if epidermal nuclear reactions are a true phenomenon or an artifact of tissue processing.
  • To investigate the mechanism of IgG penetration into epidermal nuclei.

Main Methods:

  • In-vivo and in-vitro organ-culture studies of skin explants.
  • Direct immunofluorescence for IgG and other immunoglobulins.
  • Serum absorption studies with extractable nuclear antigen (ENA).
  • Investigation of factors affecting antinuclear antibody (ANA) penetration.

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Main Results:

  • Epidermal nuclear reactions for IgG were observed, but not for other immunoglobulins, despite the presence of various ANA classes.
  • A statistically significant association was found between in-vitro epidermal nuclear reactions and serum RNP antibodies.
  • ENA absorption reduced nuclear reactivity in tissue explants.
  • ANA penetration into skin explant nuclei was time/temperature-dependent and inhibited by sodium azide and oligomycin.

Conclusions:

  • Epidermal nuclear staining by direct immunofluorescence on skin biopsies is a selective process.
  • IgG penetration into epidermal cell nuclei is an active, energy-dependent process, not an artifact.