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Related Experiment Videos

Experimental murine hypersensitivity pneumonitis.

M Schuyler1, K Gott, P Haley

  • 1Department of Medicine, Albuquerque VA Medical Center, New Mexico 87108.

Cellular Immunology
|September 1, 1991
PubMed
Summary
This summary is machine-generated.

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This study establishes a mouse model for experimental hypersensitivity pneumonitis (EHP). Adoptive transfer of specific immune cells successfully induced EHP, demonstrating its feasibility.

Area of Science:

  • Immunology
  • Pulmonary Medicine
  • Experimental Pathology

Background:

  • Hypersensitivity pneumonitis (EHP) is an immune-mediated lung disease.
  • Understanding the mechanisms of EHP pathogenesis is crucial for developing effective treatments.
  • Genetic factors may influence susceptibility and response to EHP.

Purpose of the Study:

  • To develop a reproducible mouse model of experimental hypersensitivity pneumonitis (EHP).
  • To investigate the role of genetic background in the pulmonary inflammatory response to Micropolyspora faeni.
  • To determine the feasibility of adoptive transfer of EHP.

Main Methods:

  • Established an EHP model in C57BL/6, SJL/J, and C3H/HeJ mice using Micropolyspora faeni.
  • Administered intratracheal injections of M. faeni and transferred immune cells (lymph node cells, peritoneal exudate cells, spleen cells) from sensitized donors.

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  • Challenged recipient mice with M. faeni or saline post-cell transfer.

    • Main Results:

    • Developed a functional mouse model of EHP with dose-dependent pulmonary inflammation.
    • Found no significant difference in inflammation extent across mouse strains with direct M. faeni challenge.
    • Adoptive transfer of cultured immune cells, particularly peritoneal exudate cells and lymph node cells, induced EHP in C3H/HeJ mice.

    Conclusions:

    • Experimental hypersensitivity pneumonitis (EHP) can be successfully modeled in mice.
    • Adoptive transfer of specific immune cells is a viable method to induce EHP.
    • Genetic background influences the susceptibility to adoptive transfer of EHP.