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Related Experiment Video

Updated: Jul 16, 2026

Identification of Protein Complexes in Escherichia coli using Sequential Peptide Affinity Purification in Combination with Tandem Mass Spectrometry

Identification of Protein Complexes in Escherichia coli using Sequential Peptide Affinity Purification in Combination with Tandem Mass Spectrometry

Published on: November 12, 2012

The phosphate pool in Escherichia coli THU.

S R Pai¹, H E Kubitschek

¹Biological, Environmental, and Medical Research Division, Argonne National Laboratory, IL.

FEMS Microbiology Letters

|June 1, 1991

Summary

The phosphate pool in Escherichia coli remains constant regardless of cell age. This study quantifies the relative phosphate pool size, showing stability across the bacterial cell cycle.

Area of Science:

Microbiology
Cell Biology
Biochemistry

Background:

Phosphate is an essential nutrient for bacterial growth and metabolism.
Understanding cellular phosphate dynamics is crucial for comprehending bacterial physiology.

Purpose of the Study:

To determine the phosphate pool size in Escherichia coli.
To investigate the relationship between cell age and phosphate pool size.

Main Methods:

Quantification of the intracellular phosphate pool.
Analysis of phosphate levels relative to total cell phosphate.

Main Results:

The relative phosphate pool size in Escherichia coli was determined.
This pool size was found to be independent of cell age.

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Assaying for Inorganic Polyphosphate in Bacteria

Assaying for Inorganic Polyphosphate in Bacteria

Published on: January 21, 2019

Using Microtiter Dish Radiolabeling for Multiple In Vivo Measurements Of Escherichia coli (p)ppGpp Followed by Thin Layer Chromatography

Using Microtiter Dish Radiolabeling for Multiple In Vivo Measurements Of Escherichia coli (p)ppGpp Followed by Thin Layer Chromatography

Published on: June 4, 2019

Related Experiment Videos

Last Updated: Jul 16, 2026

Identification of Protein Complexes in Escherichia coli using Sequential Peptide Affinity Purification in Combination with Tandem Mass Spectrometry

Identification of Protein Complexes in Escherichia coli using Sequential Peptide Affinity Purification in Combination with Tandem Mass Spectrometry

Published on: November 12, 2012

Assaying for Inorganic Polyphosphate in Bacteria

Assaying for Inorganic Polyphosphate in Bacteria

Published on: January 21, 2019

Using Microtiter Dish Radiolabeling for Multiple In Vivo Measurements Of Escherichia coli (p)ppGpp Followed by Thin Layer Chromatography

Using Microtiter Dish Radiolabeling for Multiple In Vivo Measurements Of Escherichia coli (p)ppGpp Followed by Thin Layer Chromatography

Published on: June 4, 2019

Conclusions:

Cellular phosphate levels in Escherichia coli exhibit stability throughout the cell cycle.
Bacterial phosphate homeostasis is maintained irrespective of cell age.