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Related Experiment Videos

The porcine tumor necrosis factor-encoding genes: sequence and comparative analysis.

P Kuhnert1, C Wüthrich, E Peterhans

  • 1Institute of Veterinary Virology, University of Berne, Switzerland.

Gene
|June 30, 1991
PubMed
Summary

Researchers cloned and sequenced porcine tumor necrosis factor-alpha and -beta genes. These genes are tandemly arranged and highly conserved with human homologues, indicating a similar evolutionary rate.

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Area of Science:

  • Genomics
  • Molecular Biology
  • Immunology

Background:

  • Tumor necrosis factor (TNF) plays a crucial role in immune responses and inflammation.
  • Understanding the genetic organization and evolution of TNF genes is vital for comparative immunology.

Purpose of the Study:

  • To clone and sequence the genomic locus of porcine tumor necrosis factor-alpha (TNF-alpha) and TNF-beta (TNF-beta) genes.
  • To investigate the arrangement and evolutionary conservation of these genes in pigs.

Main Methods:

  • Construction and screening of a porcine liver genomic DNA library using phage lambda EMBL4.
  • Hybridization with a porcine TNF-alpha cDNA probe to identify positive clones.
  • DNA sequencing of the cloned fragment.

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Main Results:

  • A 10.22-kb genomic fragment containing both porcine TNF-alpha and TNF-beta genes was successfully cloned and sequenced.
  • The genes were found to be arranged in a tandem repeat, similar to homologous genes in other species.
  • Approximately 2 kb of repetitive sequences were identified upstream of the TNF-beta gene.
  • Porcine TNF genes showed an 80% conservation with their human counterparts.

Conclusions:

  • The porcine TNF-alpha and TNF-beta genes are tandemly organized.
  • The high degree of sequence conservation suggests a conserved evolutionary rate for these genes between pigs and humans.