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Determination of diclofenac in plasma using a fully automated analytical system combining liquid-solid extraction
A Sioufi1, J Richard, P Mangoni
1Laboratoires Ciba-Geigy, Biopharmaceutical Research Centre, Rueil-Malmaison, France.
Abstract:
A fully automated analytical system based on liquid-solid extraction combined with column liquid chromatography is described for the determination of diclofenac in plasma. After addition of pH 5 buffer and the internal standard solution to the plasma sample, both sample preparation via a C18 disposable extraction column and injection were performed by a Gilson ASPEC system. Diclofenac and the internal standard were separated on a reversed-phase column, using methanol-pH 7.2 phosphate buffer (56:44, v/v) as mobile phase at a flow-rate of 0.4 ml/min. The reproducibility and accuracy of the method were acceptable over the concentration range 31-3140 nmol/l in plasma.