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Updated: Aug 9, 2026

Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid
Published on: December 4, 2017
High-performance liquid chromatographic analysis of free palmitic and stearic acids in cerebrospinal fluid
I Zamir1, E Grushka, G Cividalli
1Department of Inorganic and Analytical Chemistry, Hebrew University, Jerusalem, Israel.
Abstract:
A relatively simple method for extraction of free fatty acids from cerebrospinal fluid with aminopropyl bonded-phase columns, and the estimation of palmitic acid (C16:0) and stearic acid (C18:0) concentrations by high-performance liquid chromatographic analysis is described. The values of C16:0 and C18:0 in patients with non-neurological disorders lie within a narrow range, with a mean (+/- S.D.) of 4.02 +/- 0.33 micrograms/ml for C16:0 and 2.72 +/- 0.39 micrograms/ml for C18:0.
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