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Pirmenol determination by high-performance liquid chromatography.

G L Hoyer1

  • 1Department of Internal Medicine, College of Medicine, University of Arizona, Tucson 85724.

Journal of Chromatography
|April 19, 1991
PubMed
Summary
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This study presents a new method for measuring pirmenol in serum using high-performance liquid chromatography. The validated analytical method is accurate, sensitive, and suitable for clinical and research applications.

Area of Science:

  • Analytical Chemistry
  • Clinical Chemistry

Background:

  • Accurate quantification of drugs in biological matrices is crucial for therapeutic drug monitoring and clinical research.
  • Existing methods may lack the sensitivity, selectivity, or throughput required for routine analysis.

Purpose of the Study:

  • To develop and validate a robust analytical method for the determination of pirmenol in human serum.
  • To establish a sensitive, selective, and accurate assay for pirmenol quantification.

Main Methods:

  • Serum samples were extracted using methylene chloride at alkaline pH.
  • Pirmenol and chlorodisopyramide (internal standard) were analyzed via high-performance liquid chromatography (HPLC).
  • A C18 reversed-phase column with a mobile phase of 0.01 M K2HPO4 (pH 2.4)-acetonitrile (94:6, v/v) was employed, with detection at 210 nm.

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Main Results:

  • The HPLC method demonstrated low intra- and inter-assay variation, indicating high precision.
  • No interfering substances were observed during the analysis of patient samples and therapeutic drug mixtures.
  • The method proved to be stable, accurate, selective, and sensitive.

Conclusions:

  • The developed HPLC method is suitable for the reliable determination of pirmenol in serum.
  • This assay can be effectively utilized for analyzing multiple samples in clinical and research settings.
  • The method's robustness supports its application in therapeutic drug monitoring and pharmacokinetic studies.