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Cryoprotection of human oocytes: inappropriate exposure to DMSO reduces fertilization rates
S J Pickering1, P R Braude, M H Johnson
1Department of Anatomy, Cambridge, UK.
Human Reproduction (Oxford, England)
|January 1, 1991
Abstract:
Human oocytes were exposed to the cryoprotectant dimethyl-sulphoxide (DMSO) at either 4 or 37 degrees C. Subsequent fertilization of these oocytes showed that exposure to DMSO at 37 degrees C was associated with a greatly reduced fertilization rate when compared to untreated control oocytes, whereas no such reduction was seen in oocytes exposed to DMSO at 4 degrees C. The significance of these results for the potential cryopreservation of human oocytes is discussed.