Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...

The Moon orbits around the Earth. In turn, the Earth (and other planets) orbit the Sun. The space directly above our atmosphere is filled with artificial satellites in orbit. One can examine the circular orbit, the simplest kind of orbit, to understand the relationship between the speed and the period of planets and satellites with respect to their positions and the bodies that they orbit.
Nicolaus Copernicus (1473-1543) first suggested that the Earth and all other planets orbit the Sun in...

An LC circuit consists of an inductor and a capacitor, either in series or parallel. Consider a charged capacitor connected with an inductor in series. Before the switch is closed, all the energy of the circuit is stored in the electric field of the capacitor. When the switch is closed, the capacitor begins to discharge, producing a current in the circuit. The current, in turn, creates a magnetic field in the inductor. Because of the induced emf in the inductor, the current cannot change...

Mohr's circle is a graphical method for identifying the state of stress at a point in a material, making it easier to analyze stress transformations under plane stress conditions. This two-dimensional technique visualizes both normal and shearing stresses on an element.
Consider a set of Cartesian coordinates. The horizontal and vertical axes correspond to normal stress (σ) and shearing stress (τ), respectively. Two points, points A and B, are defined by the normal and shear stresses on the...

Mohr's circle is a crucial graphical method used to analyze plane strain by plotting strain on a set of cartesian coordinates, where the abscissa is normal strain ∈ and the ordinate is shear strain γ. Similarly to Mohr’s circle for plane stress, two points X and Y are plotted. Their coordinates are (∈x, -γXY) and (∈Y, γXY), respectively.
Mohr's circle visually represents the strain states under various conditions, which is essential for understanding material behavior. The center of Mohr's...

A circle in the coordinate plane is defined as the set of all points that lie at a constant distance, known as the radius, from a fixed point called the center. This relationship is captured using the distance formula. For a point (x, y) on the circle and a center (h, k), the distance between them equals the radius r. By squaring both sides of the distance formula, the equation of the circle is written in standard form:Constructing the Equation from Geometric InformationIf the center and the...