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Related Experiment Video

Updated: Jul 2, 2026

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
07:50

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Published on: September 18, 2020

SPMSP: a solid-phase PCR with colorimetric read-out for quantitative methylation analysis.

Sonja Sievers1, Carla Fritzsch, Cornelius Kuhnen

  • 1CMax Planck Institute of Molecular Physiology, Dortmund, Germany. sonja.sievers@mpi-dortmund.mpg.de

Anticancer Research
|August 30, 2008
PubMed
Summary
This summary is machine-generated.

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A new method, solid-phase methylation-specific PCR (SPMSP), offers high-throughput, low-cost DNA methylation analysis for cancer research. This technique quantifies DNA methylation using standard equipment, aiding cancer management.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Biomarker Discovery

Background:

  • DNA methylation is a crucial diagnostic biomarker in cancer management.
  • There is a need for high-throughput, cost-effective methods for quantitative DNA methylation detection.
  • Accurate methylation analysis is vital for advancing cancer diagnostics and treatment strategies.

Purpose of the Study:

  • To introduce and validate the solid-phase methylation-specific PCR (SPMSP) method.
  • To enable quantitative DNA methylation analysis using accessible laboratory equipment.
  • To demonstrate the application of SPMSP in analyzing colorectal carcinoma samples.

Main Methods:

  • Solid-phase methylation-specific PCR (SPMSP) combines solid-phase amplification with ELISA-based colorimetric detection.

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Last Updated: Jul 2, 2026

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
07:50

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Published on: September 18, 2020

Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

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Published on: February 24, 2015

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12:34

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Published on: October 21, 2011

  • Utilizes standard laboratory equipment, including a thermocycler and an ELISA reader.
  • Quantifies DNA methylation in specific gene promoter regions, such as the APC gene.

    • Main Results:

    • SPMSP provides a quantitative analysis of DNA methylation.
    • The method was successfully applied to quantify DNA methylation in the APC gene promoter in colorectal carcinoma samples.
    • SPMSP is compatible with standard laboratory equipment, making it broadly accessible.

    Conclusions:

    • SPMSP offers a versatile platform for DNA methylation profiling in cancer research and management.
    • The method is adaptable for analyzing other promoter sequences and can be scaled for high-throughput applications.
    • SPMSP has widespread potential for improving cancer diagnostics and therapeutic strategies.