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Alternative immunofluorescent labeling of Cryptosporidium parvum in water samples using semiconductor quantum dots

Lai Yoke Lee1, Jiang Yong Hu, Say Leong Ong

  • 1Division of Environmental Science and Engineering, National University of Singapore, Singapore.

Water Environment Research : a Research Publication of the Water Environment Federation
|August 30, 2008
PubMed

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A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
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