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Analysis of meloxicam by high-performance liquid chromatography with cloud-point extraction
1College of Pharmacy, Chosun University, 501-759, Gwangju, Korea.
Analytical and Bioanalytical Chemistry
|August 30, 2008
Summary
A new cloud-point extraction method offers a simple way to determine meloxicam in human serum. This technique provides higher recoveries than traditional liquid-liquid extraction, making it suitable for clinical analysis.
Area of Science:
- Analytical Chemistry
- Pharmaceutical Analysis
Background:
- Meloxicam is a widely used nonsteroidal anti-inflammatory drug (NSAID).
- Accurate determination of meloxicam in biological matrices like human serum is crucial for therapeutic drug monitoring and pharmacokinetic studies.
Purpose of the Study:
- To develop and validate a simple, efficient cloud-point extraction (CPE) method for meloxicam determination in human serum.
- To compare the performance of the developed CPE method with conventional liquid-liquid extraction (LLE).
Main Methods:
- Cloud-point extraction using Triton X-114, HCl, and NaCl.
- Enrichment of meloxicam with acetonitrile.
- Separation using a C18 analytical column and UV detection at 360 nm.
- Tenoxicam as an external standard.
Main Results:
- Linearity was observed in the range of 45-2000 ng/mL.
- Recoveries exceeded 92.0%, with relative error within +/-3.0%.
- The CPE method demonstrated a considerable limit of quantification (LOQ) and higher recoveries compared to LLE, although with slightly lower precision.
Conclusions:
- The developed cloud-point extraction method is a viable and efficient technique for meloxicam determination in human serum.
- This method offers advantages in terms of recovery and simplicity for clinical applications.
- The study successfully applied the method to determine meloxicam levels in healthy human volunteers.
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