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Expression of human parainfluenza virus type 3 PD protein and intracellular localization in virus infected cells

Greg Wells1, Achut Malur

  • 1Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Biotech 124, 600 Moye Boulevard, Greenville, NC 27834, USA.

Virus Genes
|August 30, 2008
PubMed

Insights

Researchers identified the PD protein in human parainfluenza virus type 3 (HPIV 3) infected cells. This finding advances understanding of viral replication and potential therapeutic targets.

Area of Science:

  • Virology
  • Molecular Biology
  • Immunology

Background:

  • Human parainfluenza virus type 3 (HPIV 3) P gene produces multicistronic mRNA.
  • P and C proteins are translated from unedited mRNA; V and PD proteins are presumed products of transcriptional editing.
  • The existence and function of HPIV 3 V and PD proteins in infected cells are not well-established.

Purpose of the Study:

  • To investigate the presence and cellular localization of the PD protein in HPIV 3 infected cells.
  • To generate tools for studying the PD protein, including in vitro expression and specific antibodies.

Main Methods:

  • In vitro expression of full-length PD protein from modified P mRNA.
  • Generation of a polyclonal antibody targeting the PD protein's COOH-terminus.
  • Confocal immunofluorescence microscopy with Leptomycin B (LMB) treatment.

Main Results:

  • Successful in vitro expression of full-length PD protein.
  • Demonstrated the presence of PD protein in both nuclear and cytoplasmic compartments of HPIV 3 infected cells.
  • Indicated the involvement of a nuclear localization signal in PD protein transport.

Conclusions:

  • The PD protein is present in HPIV 3 infected cells and exhibits nuclear-cytoplasmic shuttling.
  • These findings support further research into the role of PD protein in HPIV 3 replication.
  • The generated antibody and expression system will facilitate future studies on PD protein function.

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