Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Stochastic models indicate rapid smallpox spread and mass mortality of Indigenous Australians after colonial exposure.

Nature human behaviour·2026
Same author

An uncommon skin manifestation after radial artery catheterization.

Cleveland Clinic journal of medicine·2026
Same author

Protocol for assessment of pain biomarkers in orthopaedic patients undergoing total knee arthroplasty in a tertiary care hospital and neuroimmunopharmacology laboratory in South Australia: a cross-sectional matched-subject observational study.

BMJ open·2026
Same author

Adult Congenital Heart Disease Workforce Challenges in the United States: Current State and Future Needs: Proceedings from the American College of Cardiology (ACC), Adult Congenital Pediatric Cardiology (ACPC) Council Adult Congenital Heart Disease Summit.

JACC. Advances·2026
Same author

Joint Reaction Forces Decrease Following Total Knee Arthroplasty up to 12-Months Post-Surgery.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society·2026
Same author

Direct anterior approach for total hip arthroplasty with patients in the lateral decubitus vs supine position: A systematic review and meta-analysis.

Journal of orthopaedics·2026

Related Experiment Video

Updated: Jul 2, 2026

Multicolor Flow Cytometry-based Quantification of Mitochondria and Lysosomes in T Cells
06:22

Multicolor Flow Cytometry-based Quantification of Mitochondria and Lysosomes in T Cells

Published on: January 9, 2019

MIFlowCyt: the minimum information about a Flow Cytometry Experiment.

Jamie A Lee1, Josef Spidlen, Keith Boyce

  • 1Department of Pathology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA.

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
|August 30, 2008
PubMed
Summary

A new standard, Minimum Information about a Flow Cytometry Experiment (MIFlowCyt), ensures experimental reproducibility. This standard facilitates the clear interpretation and reuse of flow cytometry data by researchers worldwide.

More Related Videos

Characterization of Aquatic Biofilms with Flow Cytometry
08:30

Characterization of Aquatic Biofilms with Flow Cytometry

Published on: June 6, 2018

A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4
06:56

A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4

Published on: March 10, 2018

Related Experiment Videos

Last Updated: Jul 2, 2026

Multicolor Flow Cytometry-based Quantification of Mitochondria and Lysosomes in T Cells
06:22

Multicolor Flow Cytometry-based Quantification of Mitochondria and Lysosomes in T Cells

Published on: January 9, 2019

Characterization of Aquatic Biofilms with Flow Cytometry
08:30

Characterization of Aquatic Biofilms with Flow Cytometry

Published on: June 6, 2018

A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4
06:56

A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4

Published on: March 10, 2018

Area of Science:

  • Biomedical research
  • Data science
  • Scientific publishing

Background:

  • Reproducibility is a cornerstone of scientific research, enabling validation and refutation of published findings.
  • Minimum data standards are crucial for unambiguous interpretation of experimental results, benefiting data providers, users, and publishers.
  • Flow cytometry (FCM) experiments generate complex data that requires standardized reporting for effective interpretation.

Purpose of the Study:

  • To introduce the Minimum Information about a Flow Cytometry Experiment (MIFlowCyt) standard.
  • To establish a universal benchmark for reporting flow cytometry experiments.
  • To enhance the interpretability and reusability of FCM data.

Main Methods:

  • Convened a diverse international collaborative group of experts including bioinformaticians, statisticians, software developers, instrument manufacturers, and researchers.
  • Developed the MIFlowCyt standard through collaborative efforts.
  • Vetted the standard by the International Society for Advancement of Cytometry (ISAC) Data Standards Task Force, Standards Committee, membership, and Council.

Main Results:

  • The MIFlowCyt standard specifies minimum information required for reporting FCM experiments.
  • Recommendations cover specimen and reagent descriptions, instrument configuration, and data processing methods.
  • MIFlowCyt has been officially adopted as a standard by ISAC.

Conclusions:

  • The MIFlowCyt standard promotes transparency and consistency in reporting flow cytometry experiments.
  • Widespread adoption by the scientific and publishing communities will improve the understanding and reuse of FCM data.
  • This standardization effort supports the core principles of scientific validation and data sharing.