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Related Experiment Video

Updated: Jul 2, 2026

Robust Generation of Hepatocyte-like Cells from Human Embryonic Stem Cell Populations
05:49

Robust Generation of Hepatocyte-like Cells from Human Embryonic Stem Cell Populations

Published on: October 26, 2011

MAPEG expression in mouse embryonic stem cell-derived hepatic tissue system.

Dan-Yan Zhu1, Yue Du, Xin Huang

  • 1Institute of Pharmacology & Toxicology and Biochemical Pharmaceutics, Zhejiang University, Hangzhou, China.

Stem Cells and Development
|August 30, 2008
PubMed
Summary

Mouse embryonic stem cell-derived hepatic tissue shows developmental-dependent expression of membrane-associated proteins in eicosanoid and glutathione metabolism (MAPEG) superfamily members. Not all MAPEG proteins were detected, impacting understanding of inflammation and toxic events.

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Area of Science:

  • Hepatology
  • Stem Cell Biology
  • Biochemistry

Background:

  • Membrane-associated proteins in eicosanoid and glutathione metabolism (MAPEG) are crucial for inflammation and cell protection.
  • Mouse embryonic stem (ES) cell-derived hepatic tissue offers an in vitro model for studying liver development and function.

Purpose of the Study:

  • To investigate the expression patterns of MAPEG superfamily members during the differentiation of mouse ES cells into hepatic tissue.
  • To assess the functional relevance of detected MAPEG proteins in the derived hepatic tissue.

Main Methods:

  • Gene and protein expression analysis of MAPEG members in ES cell-derived hepatic tissue at various differentiation stages.
  • Enzyme activity assays for microsomal glutathione S-transferase 1 (MGST1) in the derived hepatic tissue.

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Last Updated: Jul 2, 2026

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05:49

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Published on: October 26, 2011

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  • Investigation of MGST1 dimer formation under oxidative stress.

    • Main Results:

    • All MAPEG members exhibited developmental-dependent gene expression.
    • Microsomal glutathione S-transferase 1 (MGST1) protein expression and activity were detected, but its dimerization was impaired by reactive nitrogen species.
    • Weak expression of leukotriene C(4) synthase (LTC(4)S) and microsomal prostaglandin E synthase 1 (mPGES-1) was observed, while 5-Lipoxygenase activating protein (FLAP) showed stable expression.
    • MGST2 and MGST3 proteins were not expressed despite detectable mRNA.

    Conclusions:

    • ES cell-derived hepatic tissue displays characteristic MAPEG gene expression, but protein expression is incomplete.
    • This model provides insights into the nature of ES cell-derived liver tissue and its potential for bioartificial liver systems.
    • The model is valuable for researching inflammation and toxic events in hepatology.