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[Construction and identification of Ksp-cadherin-Gpx1-Klk1 expression vector]
Li-yi Xie1, Wu-jun Xue, He-li Xiang
1Department of Kidney Transplantation, First Affiliated Hospital, Xi'an Jiaotong University College of Medicine, Xi'an, China. xieliyi0313@163.com
Objective:
To construct a Gpx1 and klk1 recombinant vector containing the kidney-specific promoter Ksp-cadherin.
Methods:
Human Gpx1, Klk1 and Ksp-cadherin cDNAs were amplified with PCR and inserted in a stepwise manner into the expressive vector pIRES-EGFP to construct the recombinant vector Ksp-cadherin-Gpx1-Klk1. The constructed vector was verified with restriction enzyme digestion and sequence analysis.
Results And Conclusion:
The recombinant expression vector Ksp-cadherin-Gpx1-Klk1 was constructed and identified successfully, which provides a potent tool for preparing transgenic animals to investigate gene therapy for ischemia-reperfusion injury in kidney transplantation.
