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Updated: Jul 2, 2026
Establishment of a Clinic-based Biorepository
Published on: May 29, 2017
[Expression of MMP1 mRNA in oral squamous cell carcinoma and paired normal tissues]
Xiao-zhi Lü1, Ya-guang Zou, Xue-min Yin
1Department of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou, China. lxzsurgeon@126.com
Objective:
To investigate the mRNA expression of matrix metalloproteinase 1 (MMP1) gene in oral squamous cell carcinoma (OSCC) and the paired normal tissues.
Methods:
The differential expression of MMP1 mRNA between 30 OSCC and paired normal tissues were detected with reverse transcription-PCR (RT-PCR).
Results:
The relative expression level of MMP1 mRNA in the OSCC tissues showed a 3.26-fold increase in comparison with that in the paired normal tissues (4.06-/+0.52 vs 1.24-/+0.17, P<0.0001). In the 30 OSCC tissues, the relative expression level of MMP1 mRNA was higher in histological grade II/III tissues (4.31-/+0.68) than in grade I (3.87-/+0.57) tissues, higher in OSCC in advanced stages (III/IV) than in tumors in early stages (I/II) (4.18-/+0.67 vs 3.65-/+0.53), and also higher in OSCC with cervical lymph node invasion than in those without cervical lymph node invasion (4.32-/+0.71 vs 3.91-/+0.51), but these differences were not statistically significant (P>0.05).
Conclusion:
MMP1 gene may play a role in local invasion of OSCC, and can serve as a potential biomarker molecule for diagnosis, treatment and prognostic evaluation of OSCC, with also clinical value for OSCC classification.
Insights
Matrix metalloproteinase 1 (MMP1) mRNA expression is significantly elevated in oral squamous cell carcinoma (OSCC) tissues compared to normal tissues. This suggests MMP1 may be a potential diagnostic and prognostic biomarker for OSCC.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Context:
- Oral squamous cell carcinoma (OSCC) is a prevalent malignancy.
- Understanding the molecular mechanisms underlying OSCC progression is crucial for improving patient outcomes.
- Matrix metalloproteinases (MMPs) are implicated in cancer invasion and metastasis.
Purpose:
- To quantify the messenger RNA (mRNA) expression levels of the matrix metalloproteinase 1 (MMP1) gene in OSCC tissues.
- To compare MMP1 mRNA expression between OSCC and adjacent normal oral tissues.
- To explore the correlation between MMP1 expression and clinicopathological features of OSCC.
Summary:
- Reverse transcription-polymerase chain reaction (RT-PCR) was employed to analyze MMP1 mRNA expression in 30 pairs of OSCC and normal tissues.
- A significant 3.26-fold increase in MMP1 mRNA levels was observed in OSCC tissues compared to normal tissues (P<0.0001).
- While higher MMP1 expression trended with advanced histological grade, stage, and lymph node invasion, these differences did not reach statistical significance.
Impact:
- The MMP1 gene may contribute to the local invasion of OSCC.
- MMP1 shows potential as a biomarker for the diagnosis, treatment, and prognostic assessment of OSCC.
- Elevated MMP1 expression could hold clinical value for classifying OSCC.
