Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Voltammetric Techniques: Cyclic Voltammetry

Voltammetric Techniques: Cyclic Voltammetry

Cyclic voltammetry (CV) is an electrochemical technique used to investigate the redox properties of a chemical species. It involves measuring the current response of an electrochemical cell as a function of the applied potential. The setup for cyclic voltammetry typically consists of a working electrode, a reference electrode, and a counter electrode—all immersed in an electrolyte solution. The working electrode is where the redox reaction of interest occurs, while the reference electrode...

Voltammetric Techniques: Linear-Scan (E vs Time)

Voltammetric Techniques: Linear-Scan (E vs Time)

Polarography is a classical voltammetric technique used to analyze electrochemical reactions. This method applies a linear potential sweep to a dropping mercury electrode (DME), and the resulting current is measured. A dropping mercury electrode is commonly used as the working electrode in polarography. It consists of a capillary tube filled with mercury, where the tiny droplet forms at the tip. This droplet continuously drops from the capillary, creating a new electrode surface for each...

Potentiometry: Membrane Electrodes

Potentiometry: Membrane Electrodes

Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at the...

Controlled-Potential Coulometry: Electrolytic Methods

Controlled-Potential Coulometry: Electrolytic Methods

Controlled-potential coulometry, also known as potentiostatic coulometry, employs a three-electrode system in which the working electrode's potential is precisely regulated using a potentiostat. Platinum working electrodes are utilized for positive potentials, while mercury pool electrodes are favored for extremely negative potentials. The platinum counter electrode is separated from the analyte using a membrane or salt bridge to avoid interference in the analysis.
The chosen potential ensures...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Peripheral blood CD8 + T-cell count at diagnosis predicts prognosis in R-CHOP-treated diffuse large B-cell lymphoma patients.

Annals of hematology·2026

Same author

Characterization of the complete chloroplast genome of <i>Bupleurum komarovianum</i> Lincz. 1950 (Apiaceae).

Mitochondrial DNA. Part B, Resources·2026

Same author

A new lungfish (Ceratodontoidei, Dipnoi) from the Early Jurassic of Chongqing, China.

Scientific reports·2026

Same author

Insights into infraspecific differentiation of the medicinally important species Bupleurum Chinense revealed by morphological and molecular evidence.

BMC plant biology·2025

Same author

Dynamics of chemical profile and microbial community in 3 consecutive years reveal Rhodococcus and Apiotrichum are potential microbes contributing to quality formation of Guang Chenpi.

Food chemistry·2025

Same author

Genetically and chemically intraspecific variations of Ficus hirta provide novel insights into its protection and utilization.

BMC plant biology·2025

Search research articles

Related Experiment Video

Updated: Jul 2, 2026

A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites

A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites

Published on: March 1, 2018

[Selective determination of norepinephrine by a cyclic voltammetric method using poly-proline-modified electrodes].

Xin-ying Ma¹, Zhi Chao

¹Department of Chemistry and Chemical Engineering, Heze University, Heze, China. lzhaojie@sohu.com

Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University

|August 30, 2008

Summary

A new proline-modified electrode accurately detects norepinephrine (NE) even with interfering substances like epinephrine and ascorbic acid. This electrochemical method offers a sensitive and selective approach for NE quantification in samples.

More Related Videos

Time-Resolved In Vivo Measurement of Neuropeptide Dynamics by Capacitive Immunoprobe in Porcine Heart

Time-Resolved In Vivo Measurement of Neuropeptide Dynamics by Capacitive Immunoprobe in Porcine Heart

Published on: May 19, 2022

Gold Nanoparticle Modified Carbon Fiber Microelectrodes for Enhanced Neurochemical Detection

Gold Nanoparticle Modified Carbon Fiber Microelectrodes for Enhanced Neurochemical Detection

Published on: May 13, 2019

Related Experiment Videos

Last Updated: Jul 2, 2026

A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites

A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites

Published on: March 1, 2018

Time-Resolved In Vivo Measurement of Neuropeptide Dynamics by Capacitive Immunoprobe in Porcine Heart

Time-Resolved In Vivo Measurement of Neuropeptide Dynamics by Capacitive Immunoprobe in Porcine Heart

Published on: May 19, 2022

Gold Nanoparticle Modified Carbon Fiber Microelectrodes for Enhanced Neurochemical Detection

Gold Nanoparticle Modified Carbon Fiber Microelectrodes for Enhanced Neurochemical Detection

Published on: May 13, 2019

Area of Science:

Electrochemistry
Analytical Chemistry
Materials Science

Context:

Norepinephrine (NE) analysis is crucial in biological and pharmaceutical fields.
Existing methods for NE determination can be affected by interfering compounds such as epinephrine (EP) and ascorbic acid (AA).
Development of selective and sensitive electrochemical sensors is an active area of research.

Purpose:

To develop a novel electrochemical method for the selective determination of norepinephrine (NE).
To utilize a proline-modified glassy carbon electrode (GCE) for enhanced NE detection.
To validate the method's efficacy in the presence of common interfering substances.

Summary:

A poly-proline film was prepared on a glassy carbon electrode (GCE) via cyclic voltammetry.
The modified electrode exhibited distinct redox peaks for NE in phosphate buffer solution (pH 7.0).
The electrochemical sensor demonstrated a linear response to NE concentrations from 7.0 x 10(-7) to 1.1 x 10(-4) mol/L, with a detection limit of 6.0x10(-8) mol/L, even in the presence of excess EP and AA.

Impact:

The proline-modified GCE offers a sensitive and selective platform for NE quantification.
The method shows good stability and applicability for analyzing NE in injection solutions.
This work contributes to the advancement of electrochemical sensing for neurotransmitter analysis.