Antimicrobial susceptibility testing of mycoplasmas by ATP bioluminescence

The susceptibility of 72 mycoplasmas to a range of antimicrobial agents was assessed in a 6-h ATP bioluminescence system. ATP was assayed with the Amerlite Analyser. Correlation with conventionally determined MICs was excellent for erythromycin and tetracycline even at 3 h. However, for ciprofloxacin, correlation was poor unless incubation was extended to 6 h.