Oxidative DNA damage is significantly correlated with flow-mediated dilation in patients with coronary artery disease
Selen Yurdakul1, Beste Ozben, Ahmet Kaya Bilge
1Department of Cardiology, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey.
Insights
Oxidative DNA damage is elevated in coronary artery disease (CAD) patients and inversely correlates with endothelial function, specifically flow-mediated dilation (FMD). This suggests DNA damage may contribute to CAD progression.
Area of Science:
- Cardiovascular Research
- Molecular Biology
- Biochemistry
Background:
- Oxidative DNA damage is elevated in coronary artery disease (CAD) patients and correlates with disease severity.
- Endothelial dysfunction is a key factor in the atherosclerotic process.
Purpose of the Study:
- To investigate the relationship between oxidative DNA damage and endothelial function in patients with CAD.
Main Methods:
- Included 40 CAD patients and 20 healthy controls.
- Assessed endothelial function using brachial artery ultrasonography to measure flow-mediated dilation (FMD).
- Quantified DNA damage using the comet assay.
Main Results:
- CAD patients exhibited significantly higher DNA damage scores (P=0.04).
- A significant negative correlation was observed between oxidized DNA damage scores and FMD (r=-0.41, P=0.009).
- Oxidized DNA damage was independently associated with FMD (standardized beta=-0.455, P=0.009) after adjusting for covariates.
Conclusions:
- DNA damage scores are significantly inversely correlated with FMD measures in CAD patients.
- This study is the first to demonstrate a link between DNA damage scores and FMD.
Background:
Oxidative DNA damage was increased in patients with coronary artery disease (CAD) and correlated with the severity of the disease. Endothelial dysfunction plays a major role in atherosclerotic process. The aim of this study was to explore a relation between oxidative DNA damage and endothelial function in patients with CAD.
Methods:
Forty patients with CAD and 20 age- and sex-matched healthy controls were included. Endothelial function was assessed by brachial artery ultrasonography. The DNA damage was determined by comet method.
Results:
The DNA damage scores after incubation with repair enzymes were found significantly higher in the patients with CAD (P = 0.04). There was a significant negative correlation between oxidized DNA damage scores and flow-mediated dilation (FMD) measures in the patients with CAD (r = -0.41; P = 0.009). Oxidized DNA damage scores were significantly and independently associated with FMD (standardized beta = -0.455; P = 0.009) when adjusted by age, sex, smoking status, blood pressure, and cholesterol levels.
Conclusions:
The DNA damage scores were significantly inversely correlated with FMD measures. To our knowledge, this is the first study showing the presence of a relation between DNA damage scores and FMD.
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