Related Experiment Video
Updated: Jul 2, 2026
11:10
Cryopreservation of Preimplantation Embryos of Cattle, Sheep, and Goats
Published on: August 5, 2011
Cryopreservation of Vanda coerulea protocorms by encapsulation-dehydration
Nipawan Jitsopakul1, Kanchit Thammasiri, Keiko Ishikawa
1Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Cryo Letters
|August 30, 2008
Summary
Cryopreservation of Vanda coerulea protocorms was achieved using encapsulation-dehydration with a loading solution. This method successfully preserved the plantlets without morphological or ploidy variations.
Area of Science:
- Plant Biotechnology
- Cryopreservation Techniques
- Orchid Conservation
Background:
- Vanda coerulea is an orchid species facing conservation challenges.
- Effective methods for long-term storage and propagation of Vanda coerulea are crucial.
Purpose of the Study:
- To establish an efficient cryopreservation protocol for Vanda coerulea protocorms.
- To assess the viability and genetic stability of cryopreserved protocorms.
Main Methods:
- Protocorms were encapsulated in alginate with a loading solution (2 M glycerol + 0.4 M sucrose).
- Preculture in modified Vacin and Went (VW) medium with 0.7 M sucrose.
- Dehydration via sterile air-flow followed by direct plunging into liquid nitrogen.
- Thawing at 40°C for 2 minutes and subsequent culture for regrowth.
Main Results:
- The highest regrowth rate of 40% was achieved with protocorms dehydrated for 8 hours, reaching 35% water content.
- Cryopreserved protocorms showed no morphological variations compared to non-cryopreserved controls.
- Ploidy levels remained unchanged after the cryopreservation process.
Conclusions:
- Encapsulation-dehydration combined with a loading solution is an effective method for Vanda coerulea cryopreservation.
- This technique ensures the genetic integrity and viability of cryopreserved orchid protocorms.
- The developed protocol offers a valuable tool for the conservation and propagation of Vanda coerulea.
More Related Videos
Related Concept Videos
01:28Cryo-electron Microscopy
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
02:45Responses to Heat and Cold Stress
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
01:27Principles of Food Preservation
Food spoilage results from microbial growth, enzymatic activity, and environmental factors that gradually degrade the sensory, nutritional, and safety qualities of food. Preservation techniques aim to slow or halt these processes to extend shelf life and maintain product quality.A key concept in food microbiology is the microbial growth curve, which includes four phases: lag, exponential (log), stationary, and death. During the lag phase, bacteria adjust to their environment without significant...