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Method for the Assessment of Effects of a Range of Wavelengths and Intensities of Red/near-infrared Light Therapy on Oxidative Stress In Vitro
Published on: March 21, 2015
Evaluation of low-level laser therapy of osteoblastic cells
Deise A A Pires Oliveira1, Rodrigo Franco de Oliveira, Renato Amaro Zangaro
1Laboratório de Dinâmica de Compartimentos Celulares, Instituto de Pesquisa e Desenvolvimento, UNIVAP, São José dos Campos, São Paulo, Brazil.
Objective:
The purpose of the present study was to evaluate the effect of biomodulation on osteoblastic cells using a gallium-aluminium-arsenide diode laser.
Background Data:
Low-level laser therapy (LLLT) is a non-pharmacological therapeutic resource to which biological tissues respond well, producing such effects as the acceleration of bone formation and bone repair.
Materials And Methods:
Osteoblastic cell cultures (OFCOL II) were irradiated with a gallium-aluminium-arsenide diode laser (GaAlAs lambda = 830 nm; 50 mW; 3 J/cm(2); 600-microm-diameter optical fiber) and divided into two groups: group 1--irradiated cells, and group 2--non-irradiated cells. Irradiation occurred at 24-h intervals for a total of 3 d. After each interval, the cells were marked with Mito Tracker Orange dye to assess the biostimulatory effect on mitochondrial activity and cell proliferation using an MTT assay.
Results:
Intense grouping of mitochondria in the perinuclear region was observed at 24 h and 48 h following irradiation. Changes from a filamentous to a granular appearance in mitochondrial morphology and mitochondria distributed throughout the cytoplasm were observed 72 h following proliferation. Such changes led to an in vitro proliferation process, as confirmed by the MTT assay.
Conclusion:
LLLT has shown itself capable of altering mitochondrial activity and the population of OFCOL II cells.
