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Updated: Jul 2, 2026

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry
Published on: February 19, 2020
Sample preparation for liquid chromatography-tandem mass spectrometry using functionalized ferromagnetic
Michael Vogeser1, Albert Geiger, Rupert Herrmann
1Institute of Clinical Chemistry, Hospital of the University of Munich, Marchioninistr. 15, D-81377 Munich, Germany. Michael.Vogeser@med.uni-muenchen.de
Objectives:
To study the applicability of functionalized ferromagnetic micro-particles in the sample preparation for quantification of small molecules by LC-MS/MS.
Design And Methods:
A representative analyte (itraconazole) was extracted from plasma samples using small quantities of C18-modified ferromagnetic micro-particles.
Results:
125 microg of functionalized micro-particles was sufficient to extract the target analyte from 10 microL of plasma with a recovery rate of approximately 100%; linearity (r>0.99) and reproducibility (inter-assay CV< or =7%) of quantitative results was found.
Conclusions:
C18-functionalized ferromagnetic particles can be used to efficiently extract small molecule analytes from complex biological matrices for quantitative analyses using LC-MS/MS.

