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Published on: March 21, 2018
Au-nanoparticles as an electrochemical sensing platform for aptamer-thrombin interaction
Elena Suprun1, Victoria Shumyantseva, Tatiana Bulko
1Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Pogodinskaya Street 10, Moscow 119121, Russia.
Biosensors & Bioelectronics
|August 30, 2008
Summary
A new electrochemical method uses gold nanoparticles to detect thrombin binding. This sensitive biosensor achieves a low detection limit for thrombin, enabling precise bioaffinity interaction analysis.
Area of Science:
- Electrochemistry
- Nanomaterials Science
- Biotechnology
Background:
- Electrochemical methods offer sensitive detection of biomolecules.
- Gold nanoparticles enhance sensor performance due to their unique properties.
- Bioaffinity interactions are crucial targets in diagnostics.
Purpose of the Study:
- To develop a novel electrochemical sensing platform for bioaffinity interactions.
- To utilize gold nanoparticles and stripping voltammetry for enhanced detection.
- To establish a model system for detecting thrombin using an aptamer.
Main Methods:
- Immobilization of thrombin-binding aptamer on gold nanoparticle-modified screen-printed electrodes via avidin-biotin technology.
- Electrochemical detection based on gold surface oxidation and stripping voltammetry.
- Utilizing ferricyanide/ferrocyanide redox couple as an electrochemical indicator.
Main Results:
- A proportional relationship was observed between cathodic peak area and adsorbed thrombin quantity.
- A sigmoid calibration curve, typical for immunoassays, was obtained.
- A low detection limit of 10(-9)M for thrombin was achieved.
- A linear range from 10(-8) to 10(-5)M thrombin concentration was established.
Conclusions:
- The developed electrochemical method provides a sensitive and specific platform for detecting bioaffinity interactions.
- Gold nanoparticle-modified electrodes combined with stripping voltammetry are effective for thrombin detection.
- The method demonstrates potential for sensitive biosensing applications.
