Platelet-rich plasma induces mRNA expression of VEGF and PDGF in rat bone marrow stromal cell differentiation
ZhenMing Hu1, Sean A F Peel, Stephen K C Ho
1Department of Orthopedic Surgery, The First Affiliated Hospital, Chongqing Medical University, Chongqing, China. zhenminghu62@yahoo.com.cn
Objective:
To investigate the potentially useful of platelet-rich plasma (PRP) on mRNA expression of angiogenesis.
Study Design:
Adjunct assay and reverse-transcription polymerase chain reaction (RT-PCR) analysis of type I collagen, vascular endothelial growth factor (VEGF), and platelet-derived growth factor (PDGF) in rat bone marrow stromal cells differentiation in 14 days' culture.
Results:
The PRP significantly elevated alkaline phosphatase activity after day 5 (P < .05), and DNA and protein content increased at culture days 1, 3, and 5 (P < .01) with PRP compared with control. The RT-PCR demonstrated that type I collagen was expressed in all substrates and remained high with PRP during 14 days of culture, and that mRNA expression of VEGF and PDGF were higher over time.
Conclusions:
This study indicates a potential contribution of PRP as possibly starting the process of angiogenesis, recruiting the endothelial cells which line blood vessels, and beginning the initiation of bone regeneration.
