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Related Experiment Videos

Recipes for reconstituting skin.

E Bell1, M Rosenberg, P Kemp

  • 1Organogenesis Inc., Cambridge, Massachusetts 02142.

Journal of Biomechanical Engineering
|May 1, 1991
PubMed
Summary
This summary is machine-generated.

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Living Skin Equivalent (LSE) fabrication involves dermal equivalents (DE) and keratinocytes. Modifying collagen contraction and biosynthesis via ascorbate and TGF-β1 enhances tissue development and matrix production for LSE and LDE.

Area of Science:

  • Tissue engineering
  • Biomaterials science
  • Dermatology

Background:

  • Living Skin Equivalent (LSE) comprises a dermal equivalent (DE) with plated keratinocytes forming a differentiated epidermis.
  • The DE develops from fibroblast and collagen fibril interactions, with gel contraction influencing tissue morphology.
  • Dermal cells are biosynthetically active, influencing matrix composition based on stimulation and epidermal presence.

Purpose of the Study:

  • To investigate the impact of physical constraints and biochemical factors on dermal equivalent (DE) development and collagen biosynthesis.
  • To explore the role of ascorbate and TGF-β1 in modulating collagen synthesis and matrix production within the DE.
  • To compare the responses of reconstituted skin models (LSE, LDE) to those of monolayered cells.

Main Methods:

Related Experiment Videos

  • Fabrication of DE by casting a cell-matrix precursor and allowing collagen polymerization.
  • Application of physical constraints (securing ends/edges) during DE casting.
  • Treatment with varying concentrations and timings of ascorbate and TGF-β1 during DE culture.
  • Analysis of collagen biosynthesis, hyaluronate, and glycosaminoglycan (GAG) production.
  • Exposure of LSE and LDE to UV radiation and chemicals for response assessment.

Main Results:

  • Securing the DE alters its dimensions, strength, and morphology.
  • Ascorbate and TGF-β1 significantly increase total collagen biosynthesis in the DE.
  • Daily ascorbate application post-casting blocks contraction and enhances collagen, hyaluronate, and GAG production.
  • LSE and LDE show complex, distinct responses to UV and chemical stimuli compared to monolayer cells.

Conclusions:

  • Physical manipulation during DE casting influences tissue development.
  • Biochemical factors like ascorbate and TGF-β1 are critical regulators of DE matrix synthesis.
  • Optimized ascorbate treatment enhances DE matrix production and prevents contraction.
  • LSE and LDE models offer more complex and relevant biological responses than traditional cell cultures.