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Related Experiment Video

Updated: Jul 2, 2026

Demonstration of Self-Assembled Cell Sheet Culture and Manual Generation of a 3D Tendon/Ligament-Like Organoid by using Human Dermal Fibroblasts

Demonstration of Self-Assembled Cell Sheet Culture and Manual Generation of a 3D Tendon/Ligament-Like Organoid by using Human Dermal Fibroblasts

Published on: June 21, 2024

[In vitro tendon engineering using human dermal fibroblasts].

Dan Deng¹, Wei Liu, Feng Xu

¹Department of Plastic and Reconstructive Surgery, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, China.

Zhonghua Yi Xue Za Zhi

|September 2, 2008

Summary

This summary is machine-generated.

Human dermal fibroblasts (DFbs) can engineer tendons in vitro using polyglycolic acid (PGA) scaffolds. Mechanical stimulation aids tissue formation, but prolonged tension may be detrimental.

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Last Updated: Jul 2, 2026

Demonstration of Self-Assembled Cell Sheet Culture and Manual Generation of a 3D Tendon/Ligament-Like Organoid by using Human Dermal Fibroblasts

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Published on: June 21, 2024

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Engineering Tendon Assembloids to Probe Cellular Crosstalk in Disease and Repair

Engineering Tendon Assembloids to Probe Cellular Crosstalk in Disease and Repair

Published on: March 22, 2024

Area of Science:

Tissue Engineering
Biomaterials Science

Context:

Developing functional artificial tendons is crucial for regenerative medicine.
Human dermal fibroblasts (DFbs) offer a potential cell source for tendon tissue engineering.

Purpose:

To assess the feasibility of using DFbs on polyglycolic acid (PGA) scaffolds for in vitro tendon engineering.
To investigate the impact of mechanical stimulation (tension) on DFb-PGA constructs.

Summary:

DFbs seeded on PGA scaffolds formed neo-tendons with collagen fiber production.
Tensioned DFb-PGA constructs exhibited improved collagen alignment and biomechanical properties compared to non-tensioned controls.
Optimal tendon formation was observed with mechanical stimulation, though prolonged tension led to negative effects.

Impact:

Demonstrates the potential of DFbs for in vitro tendon regeneration.
Highlights the importance of controlled mechanical loading in tissue engineering.
Provides insights into optimizing scaffold design and stimulation parameters for tendon repair.