TaqMan-based real-time PCR method for detection of Yersinia pseudotuberculosis in food
S Thisted Lambertz1, C Nilsson, S Hallanvuo
1Research and Development Department, National Food Administration, Uppsala, Sweden.
Applied and Environmental Microbiology
|September 2, 2008
Abstract:
A sensitive and specific assay for detection of food-borne pathogenic Yersinia pseudotuberculosis was developed. The primer-probe set was designed to target a 157-bp sequence of the chromosomally located gene ail. The complete method, including an internal amplification control, was evaluated for several different food items.
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