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Related Experiment Videos

Endothelin-1-induced nociception.

R B Raffa1, J J Schupsky, R P Martinez

  • 1Drug Discovery Research, R.W. Johnson Pharmaceutical Research Institute, Spring House, PA 19477-0776.

Life Sciences
|January 1, 1991
PubMed
Summary
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Morphine administered into the brain or spinal cord reduced pain responses to endothelin-1 (ET-1) in mice. This suggests ET-1 pain signaling involves opioid pathways, offering a new visceral pain model.

Area of Science:

  • Neuroscience
  • Pharmacology
  • Pain Research

Background:

  • Endothelin-1 (ET-1) is implicated in various physiological processes.
  • The role of ET-1 in pain signaling, particularly visceral pain, requires further elucidation.
  • Opioid receptors are key modulators of pain pathways.

Purpose of the Study:

  • To investigate the effect of central morphine administration on ET-1-induced abdominal constriction in mice.
  • To determine if ET-1-induced pain is mediated by opioid pathways.
  • To establish ET-1-induced abdominal constriction as a potential novel pain model.

Main Methods:

  • Intracerebroventricular (i.c.v.) and intrathecal (i.t.) administration of morphine in mice.
  • Induction of abdominal constriction using intraperitoneal (i.p.) injection of endothelin-1 (ET-1).

Related Experiment Videos

  • Assessment of morphine's antinociceptive effects and blockade by naloxone and beta-funaltrexamine (beta-FNA).

    • Main Results:

    • Morphine administered i.c.v. or i.t. significantly antagonized ET-1-induced abdominal constriction.
    • The antinociceptive effect of morphine was dose-dependently blocked by naloxone and beta-FNA.
    • Effective doses (ED50) for i.c.v. and i.t. morphine were 39.3 ng and 1.5 ng, respectively.

    Conclusions:

    • ET-1-induced abdominal constriction is transmitted via ascending pain pathways sensitive to opioid receptor activation (mu-opioid receptors).
    • This study validates ET-1-induced abdominal constriction as a novel pain model.
    • The model shows potential utility for studying visceral pain, such as anginal pain.