Related Experiment Video
Updated: Jul 2, 2026

Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues
Published on: February 17, 2017
Optimization of trehalose production by a novel strain Brevibacterium sp. SY361
Lei Wang1, Rui Huang, Guanbin Gu
1Microbiology Department, School of Medicine, Soochow University, Suzhou, P.R. China.
Abstract:
Trehalose production by a novel strain of Brevibacterium sp. SY361 was optimized in submerged fermentation. Different chemical and physical parameters such as carbon and nitrogen sources, inoculum level, initial pH, incubation temperature, aeration and time-course of fermentation, were studied in order to increase trehalose productivity. An optimal production medium containing 3% (w/v) glucose, 0.9% (v/v) corn steep liquor, 0.5% (w/v) KH(2)PO(4) and 0.4% (w/v) MgSO(4).7 H(2)O was found suitable for trehalose production. An optimal volume of medium in a 500 ml flask was 80 ml. The optimal levels of other parameters were 4.0% (v/v) of inoculum, initial pH of 6.0, incubation temperature of 28-32 degrees C and time-course of 60 h. Optimized parameters gave a maximum trehalose of 12.2 mg/ml with a conversion rate of 58.4%.
More Related Videos
Related Concept Videos
Bioreactor Controls-III
Hyperthermophilic Bacteria
Production of Alcohol
Designing Growth Media for Bioreactors
Production of Antibiotics
Microbes in Food Production

