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Updated: Jul 2, 2026

Synthesis of a Deuterated Standard for the Quantification of 2-Arachidonoylglycerol in Caenorhabditis elegans
Published on: September 21, 2019
Membrane microdomains and metabolic pathways that define anandamide and 2-arachidonyl glycerol biosynthesis and
Ekaterina A Placzek1, Yasuo Okamoto, Natsuo Ueda
1Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, 575 Stadium Mall Drive, Room 202C, West Lafayette, IN 47904, USA.
Abstract:
Anandamide (AEA) and 2-arachidonyl glycerol (2-AG), endogenous ligands for the CB1 and CB2 cannabinoid receptors, are referred to as endocannabinoids because they mimic the actions of delta9-tetrahydrocannabinol (Delta9-THC), a plant-derived cannabinoid. The processes by which AEA and 2-AG are biosynthesized, released, taken up by cells and hydrolyzed have been of much interest as potential therapeutic targets. In this review we will discuss the progress that has been made to characterize the primary pathways for AEA and 2-AG formation and breakdown as well as the role that specialized membrane microdomains known as lipid rafts play in these processes. Furthermore we will review the recent advances made to track and detect AEA in biological matrices.
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