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Published on: July 11, 2014
Gas chromatographic separation of methoxylated polychlorinated biphenyl atropisomers
Izabela Kania-Korwel1, Sandhya M Vyas, Yang Song
1Department of Occupational and Environmental Health, University of Iowa, 124 IREH, Iowa City, IA 52242, USA.
Abstract:
Several polychlorinated biphenyls (PCBs) and their hydroxylated metabolites display axial chirality. Here we describe an enantioselective, gas chromatographic separation of methylated derivatives of hydroxylated (OH-)PCB atropisomers (MeO-PCB) using a chemically bonded beta-cyclodextrin column (Chirasil-Dex). The atropisomers of several MeO-PCBs could be separated on this column with resolutions ranging from 0.42 to 0.87 under isothermal or temperature-programmed conditions. In addition, the enantiomeric fraction of OH-PCB 136 metabolites was determined in male and female rats treated with racemic PCB 136. The methylated derivatives of two OH-PCB 136 metabolites showed an enantiomeric enrichment in liver tissue, whereas PCB 136 itself was near racemic.
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