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A DNA sensor based on surface plasmon resonance for apoptosis-associated genes detection
Wei Jin1, Xiaochen Lin, Shaowu Lv
1Research Center for Analytical Instrumentation, State Key Lab of Industrial Control Technology, Institute of Cyber-Systems and Control, College of Information Science and Engineering, Zhejiang University, Hangzhou 310058, PR China.
Biosensors & Bioelectronics
|September 2, 2008
Summary
A novel DNA sensor using surface plasmon resonance (SPR) accurately detects apoptosis genes bcl-2 and bax. This sensitive and cost-effective sensor offers real-time monitoring for biological research.
Area of Science:
- Biotechnology
- Molecular Biology
- Biosensing
Background:
- Apoptosis plays a crucial role in cellular processes and disease.
- Accurate detection of apoptosis-associated genes like bcl-2 and bax is vital for research.
- Existing methods for gene detection can be complex and time-consuming.
Purpose of the Study:
- To develop a practical and simple DNA sensor for detecting apoptosis-associated genes.
- To utilize surface plasmon resonance (SPR) with multi-wavelength detection for enhanced sensitivity.
- To establish a reliable method for real-time monitoring of DNA hybridization.
Main Methods:
- Developed an SPR-based DNA sensor using biotin-avidin immobilization.
- Employed complementary oligonucleotide sequences of bcl-2 and bax as probes.
- Investigated sensor assembly, real-time monitoring of DNA immobilization and hybridization.
- Determined detection ranges for oligonucleotides and PCR products of bcl-2 and bax.
Main Results:
- The SPR sensor demonstrated real-time monitoring of DNA immobilization and hybridization.
- Established determination ranges: 50-400 ng/mL for oligonucleotides, 5-60 ng/mL for bcl-2 PCR, and 5-40 ng/mL for bax PCR.
- Sensor showed high stability (approx. 90 uses), low R.S.D. (1.2-1.3%), and specificity, with no interference from non-complementary DNA.
- Achieved simplicity, sensitivity, selectivity, rapid response, and cost-effectiveness.
Conclusions:
- The developed SPR DNA sensor is a practical and effective tool for detecting apoptosis-associated genes.
- The sensor's real-time capabilities and high performance make it suitable for studying apoptosis and signal transduction.
- This cost-effective biosensor offers a promising alternative for genetic analysis in research settings.
