Related Experiment Video
Updated: Jul 2, 2026

Design of Solid-State Fermentation Systems for Polymer Hydrolytic Extracellular Enzyme Production by Filamentous Fungi
Published on: June 6, 2025
Mixture design as first step for improved glutaminase production in solid-state fermentation by isolated Bacillus
T Sathish1, G S Lakshmi, Ch S Rao
1Bioengineering and Environmental Centre, Indian Institute of Chemical Technology, Hyderabad, India.
Aim:
Investigation of mixture-design impact on glutaminase production by isolated Bacillus sp.
Methods And Results:
An augmented simplex centroid design was used to optimize a three (wheat bran, Bengal gram husk and palm seed fibre) component mixture for glutaminase production. Selected substrate materials showed impact on glutaminase production values at individual level by Bengal gram husk [2789 U gds(-1) (gram dry substrate] and in two-level combination with wheat bran and Bengal gram husk (maximum of 3300 U gds(-1)).
Conclusion:
Bengal gram husk is the most suitable substrate medium for glutaminase production by Bacillus sp. Maximum glutaminase production is achieved using solid-substrate mixture at two-level combinations in the ratio of 66 : 34 for Bengal gram husk and wheat bran, respectively.
Significance And Impact Of The Study:
The present study has significance in large-scale production of glutaminase at commercial level with the use of multi-substrate rather than single-substrate/support material.
Related Concept Videos
Bioreactor Controls-III
Scale-Up Processes
Production of Antibiotics
Bioreactor Design and Operational System
Upstream Processing
Production of Organic Acids

