Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 2, 2026

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy
10:03

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy

Published on: June 27, 2014

Studying dynamic process of photosensitization by ESR method.

H Zhang1, H Zhao, Z Zhang

  • 1Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.

Science in China. Series C, Life Sciences
|August 1, 1997
PubMed
Summary

This study establishes a kinetic method to determine the rate constants for generating active intermediates during photosensitization. It reveals structure-activity relationships for perylenequinone photosensitizers and their solvent interactions.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Human cancer cells exhibit protein kinase C-dependent c-erbB-2 transmodulation that correlates with phosphatase sensitivity and kinase activity.

The Journal of biological chemistry·1996
Same author

Covalent linkage between proteins of the inter-alpha-inhibitor family and hyaluronic acid is mediated by a factor produced by granulosa cells.

The Journal of biological chemistry·1996
Same author

Induction of apoptosis in prostatic tumor cell line DU145 by staurosporine, a potent inhibitor of protein kinases.

The Prostate·1996
Same author

People's Republic of China: status of cancer pain and palliative care.

Journal of pain and symptom management·1996
Same author

Hybrid selection of transcribed sequences from microdissected DNA: isolation of genes within amplified region at 20q11-q13.2 in breast cancer.

Cancer research·1996
Same author

Common sequence variants of lipoprotein lipase: standardized studies of in vitro expression and catalytic function.

Biochimica et biophysica acta·1996

Area of Science:

  • Photochemistry
  • Chemical Kinetics
  • Organic Chemistry

Background:

  • Photosensitization involves generating reactive intermediates.
  • Understanding the mechanisms (Type I and Type II) is crucial for controlling these reactions.
  • Perylenequinone derivatives are potential photosensitizers.

Purpose of the Study:

  • To establish a kinetic method for quantifying active intermediate generation during photosensitization.
  • To calculate relative rate constants for singlet oxygen (1O2), superoxide (O(-)(2)), and photosensitizer radical anion (PS(-)(2)) formation.
  • To investigate the structure-activity relationships of perylenequinone photosensitizers and solvent effects on photosensitivity.

Main Methods:

  • Utilized kinetic methods and TEMPONE trapping to monitor TAN radical formation over time.

More Related Videos

Electron Spin Resonance Micro-imaging of Live Species for Oxygen Mapping
09:40

Electron Spin Resonance Micro-imaging of Live Species for Oxygen Mapping

Published on: August 26, 2010

Determination of the Photoisomerization Quantum Yield of a Hydrazone Photoswitch
09:33

Determination of the Photoisomerization Quantum Yield of a Hydrazone Photoswitch

Published on: February 7, 2022

Related Experiment Videos

Last Updated: Jul 2, 2026

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy
10:03

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy

Published on: June 27, 2014

Electron Spin Resonance Micro-imaging of Live Species for Oxygen Mapping
09:40

Electron Spin Resonance Micro-imaging of Live Species for Oxygen Mapping

Published on: August 26, 2010

Determination of the Photoisomerization Quantum Yield of a Hydrazone Photoswitch
09:33

Determination of the Photoisomerization Quantum Yield of a Hydrazone Photoswitch

Published on: February 7, 2022

  • Established a functional relationship between TAN radical concentration and time during photosensitization.
  • Employed a derived formula to calculate relative rate constants for various active intermediates.

    • Main Results:

    • Successfully established the kinetic relationship for TAN radical generation.
    • Calculated relative rate constants for 1O2, O(-)(2), and PS(-)(2) for three perylenequinone photosensitizers (HA, HB, CP).
    • Quantified the influence of solvent systems (DMF-H2O and DMSO-H2O) on photosensitizer activity.

    Conclusions:

    • The developed kinetic method allows for the calculation of rate constants for photosensitization intermediates.
    • Structure-activity relationships of perylenequinone photosensitizers were elucidated.
    • Significant solvent effects on the photosensitivity of these compounds were observed.